Reproduction Abstracts

The development of culture methods to produce oocytes from immature ovarian follicles in vitro would greatly contribute to livestock production and assisted human reproduction. However, successful in vitro culture to produce fully mature oocytes is very difficult in the case of large domestic animals. In the present study, we investigated the growth properties of in vitro-cultured porcine oocytes derived from early developmental stage follicles. Oocy...

Introduction: In general, the maternal age affects the quality of the oocytes as well as that of embryos. This study aims to examine whether changes in the follicular fluid (FF) can affect oocyte quality in aged cows.Materials and methods: Oocytes were collected from ovaries of young (<120 months) or aged cows (≥120 months), and oocytes from animals in these two age groups were cultured in a maturation medium that contained 10% FF derived from ...

Introduction: The present study aims to investigate the effect of resveratrol supplementation on the in vitro development of porcine oocytes derived from early antral follicles.Materials and methods: Oocyte-granulosa cell complexes (OGCs) were collected from early antral follicles that were 0.5–0.7 mm in diameter and cultured in the absence or present of resveratrol (2 μM) for 14 days. The effect of resveratrol on SIRT1 expression was ...

Introduction: Proliferation of granulosa cells (GCs) is essential for oocyte growth toward ovulation. Extreme proliferation of GCs is taken place under hypoxic condition due to lack of vascularization in the follicles. However, the mechanisms underlying proliferation of GCs under hypoxia have not been elucidated. In the present study, we examined the effect of low oxygen level on molecular background of cellular proliferation in bovine GCs.Methods: Bovin...